Akademik Veri Yönetim Sistemi
International Journal of Agriculture, Forestry and Life Sciences, cilt.5, sa.1, ss.87-93, 2021 (Hakemli Dergi)
With a cosmopolite widespread worldwide, Ascocyhta rabiei may result in yield losses in chickpea fields up to 100% when
the climate conditions were available for growth and development of the pathogen. In this study the effects of culture media,
temperature, pH and light regimes on mycelial growth of nine isolates of A. rabiei isolated from different agroecological zones
of Yozgat province (Turkey) were investigated. Malt Extract Agar was most suitable for mycelial growth of all nine isolates
tested. Chickpea Seed Dextrose Agar, Oat Seed Dextrose Agar and ½ Potato Dextrose Agar were not suitable for mycelial growth
of all isolates, whereas pycnidial production was observed usually on CSDA or OMA. A. rabiei isolates generally developed
smooth, sparse and immersed mycelium in all of culture media. Mycelium was characterized with pale cream, light greenish
grey or grey-black color. A. rabiei grew from 15 to 30 °C, with optimum growth at 20 °C and no growth at 35 °C. The temperature
required for maximum daily mycelial growth (2.0 mm day-1
) was identified as 20.0°C Present isolates formed smaller-diameter
colonies at acidic pH values as compared to basic pH values. The pH values of 6.0 and 7.0 promoted mycelial growth. Light
significantly influenced radial mycelial growth of YBUAr7 isolate of A. rabiei. Prolonged light exposures promoted radial
growth in culture media and the cultures incubated under continuous light exhibited greater radial growth rates than the cultures
incubated under 12 h photoperiods and 24 h dark.