Tuzcu M., Tuzcu N., Akçakavak G.
Revista Científica de la Facultad de Ciencias Veterinarias, cilt.33, sa.2, ss.1-5, 2023 (SCI-Expanded)
Özet
Brucellosis is a zoonotic disease that affects a large number of people
and animals, causing physical disability, workforce loss and significant
economic losses in the livestock industry. In the current study, it was
aimed to determine and compare the levels of tumor necrosis factor
alpha (TNF–
α
), interferon gamma (IFN–
γ
), Procalcitonin (PCT) and
Neopterin in the blood serums of cattle with brucellosis and vaccinated
against brucellosis. The materials of this study consisted of a total 48
blood serums belonging to three basic groups, each consisting of 16
animals. Disease group (1st group) were divided into two subgrups each
consisting of 8 animals that 21st day after abortion and seropositive
7 months pregnant, the vaccinated (2nd group) and the control (3rd
group) groups were divided into two subgroups, each consisting of
8 animals that gave birth 21 days ago and 7 months pregnant. IFN–
γ
and PCT levels were determined by sandwich enzyme immunoassay,
TNF–
α
and Neopterin levels were determined using competitive
inhibition enzyme immunoassay method by using ELISA device. In this
study, TNF–
α
, PCT and Neopterin levels measured in the blood serums
of the
Brucella
seropositive (1st), conjunctival
Brucella
abortus S19
vaccine administered (2nd) and unvaccinated
Brucella
seronegative
control groups were compared and no significant difference could be
determined between the subgroups of the groups (
P
>0.05). There were
a significant differences between 1st, 2nd, and 3rd groups (
P
<0.05).
IFN–
γ
levels determined in the blood serums of 1st, 2nd and 3rd groups
were compared and no
s
ignificant differences were found between the
subgroups of 2nd and 3rd groups (
P
>0.05), but there were a significant
differences between the subgroups of the 1st group (
P
<0.05). Similarly, a
significant differences were determined between 1st, 2nd and 3rd groups
in terms of IFN–
γ
levels (
P
<0.05). As a result, it was thought that detecting
very high serum TNF–
α
, IFN–
γ
, neopterin levels in cattle with brucellosis
would be helpful in the diagnosis and follow–up of brucellosis. However,
it was concluded that there is a need for controlled studies comparing
more herds with brucellosis to determine whether the relevant cytokines
can be used in the diagnosis
of brucellosis