Akademik Veri Yönetim Sistemi
ISPEC 18. ULUSLARARASI TARIM, HAYVANCILIK VE KIRSAL KALKINMA KONGRESİ, Konya, Türkiye, 24 - 26 Ekim 2025, ss.1-10, (Tam Metin Bildiri)
Tobacco (Nicotiana tabacum L.) is widely cultivated worldwide for non-food purposes. As with all crops, hybrid varieties of tobacco exhibit superior yield and quality characteristics. Certain techniques can be used to produce seeds for hybrid varieties. This study aims to share the results of hybridization using pollen obtained from the male parent through pollen harvesting methods and stored under different conditions, applied to the cytoplasmic male sterile female parent. The research was carried out in open field conditions in the Tokat-Kazova ecology in 2021-2022. The TT5 basma tobacco line, which is the paternal parent of the KINAY hybrid tobacco variety, was used as the pollen donor in the study. Anthers were collected from this line at the stage when the corolla elongated and swelled and anthocyanin accumulation began. The anthers were dried in an oven at 35 °C for 23 hours. The pollen was gently shaken through a fine sieve and stored. The viability of the pollen on the stigma and its seed-forming ability were determined using the TT2 (BC6F1) basma tobacco line, which is the maternal parent of the KINAY hybrid tobacco variety. The pollen obtained was stored in a household refrigerator at +4 °C and -20 °C. The pollen was used in hybridization procedures immediately after harvest and one year after harvest. The amount of seed obtained per capsule in the hybridizations performed at the time of pollen harvest was found to be 0.2135 g. In the hybridizations performed one year later, the amount of seed obtained per capsule from pollen stored at +4 °C was determined to be 0.1780 g, while that from pollen stored at -20 °C was 0.2000 g. Compared to the seed quantity obtained from the hybridization performed at the time of pollen harvest, a 16.63% decrease in seed quantity was observed in the hybridization performed with pollen stored at +4 °C for one year, and a 6.32% decrease was observed in the hybridization performed with pollen stored at -20 °C. It was found that -20 °C is a more suitable storage temperature than +4 °C for one-year storage. Seedlings were produced from the seeds obtained, and no physiological abnormalities were observed. Although there was a decrease in seed quantity in crossbreeding with stored pollen compared to fresh pollen, it was seen that pollen can be stored and used for long periods and provides a significant advantage in hybrid seed production. Additionally, new research is needed on the harvesting, storage, and transportation of pollen in tobacco breeding.