Therapeutic effects of apocynin on ovarian ischemia-reperfusion induced lung injury


TANYELİ A., Erdogan D. G. , ÇOMAKLI S., Polat E., GÜLER M. C. , ERASLAN E. , ...More

BIOTECHNIC & HISTOCHEMISTRY, 2022 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2022
  • Doi Number: 10.1080/10520295.2022.2036368
  • Title of Journal : BIOTECHNIC & HISTOCHEMISTRY
  • Keywords: Apocynin, apoptosis, ischemia, lung injury, oxidative damage, rats, reperfusion, GERM-CELL APOPTOSIS, ISCHEMIA/REPERFUSION INJURY, OXIDATIVE STRESS, ENDOPLASMIC-RETICULUM, AUTOPHAGY, INFLAMMATION, INHIBITION, PATHWAY, TISSUE, DEATH

Abstract

Ovarian ischemia-reperfusion (I-R) injury may damage remote organs, including the lungs. We investigated whether apocynin, a NADPH oxidase inhibitor, might protect against ovarian I-R induced apoptosis in the lungs of rats. Bilateral ovarian I-R was induced for 3 h, then apocynin was applied at two concentrations. Lung tissue was evaluated using spectrophotometric and immunohistochemical methods. We found that I-R increased total oxidant status (TOS), oxidative stress index (OSI) and myeloperoxidase (MPO) levels, and immunostaining of nuclear factor kappa-B (NF-kappa B), light chain 3B (LC3B), interleukin 1-beta (IL-1 beta), caspase-3 and tumor necrosis factor-alpha (TNF-alpha), but decreased superoxide dismutase (SOD) values. Apocynin application to I-R injured rats enhanced recovery of lung tissue oxidants and improved both histology and frequency of apoptosis.