Determination of embryotoxic effects of Tarantula cubensis alcoholic extract with in ovo model


MAGYAR ALLATORVOSOK LAPJA, vol.143, pp.497-504, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 143
  • Publication Date: 2021
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Page Numbers: pp.497-504
  • Yozgat Bozok University Affiliated: Yes


Background: Tarantula cubensis alcoholic extract (TCAE), a homeopathic product,is widely used in the treatment of different diseases in many animal species. How-ever, there is no conclusive data about its safety in pregnancy. In ovo models havefrequently been used for embryotoxicity studies. Thus, it has been hypothesizedthat the embryotoxic effects of TCAE could be determined by an in ovo model.Objectives: The aim of this research was to determine the embryotoxic effectsand lethal dose 50 (LD50) of TCA using fertile eggs with an in ovo model.Materials and Methods: A total of 360 fertile eggs were kept in an incubator andrandomly divided into 12 groups (n = 30). Egg fertility was controlled on day 7. Thefirst group of eggs was not treated. The second group was injected with 50 μL salinesolution. The next 5 groups were injected with TCAE at doses of 50 μL/egg, 25 μL/egg,12.5 μL/egg, 6.25 μL/egg and 3.125 μL/egg, respectively. Ethanol, the vehicle substanceof the commercial TCAE product, was injected at doses of 18.2 μL/egg, 9.1 μL/egg,4.6 μL/egg, 2.3 μL/egg and 1.2 μL/egg, respectively, into the remaining 5 groups. Thedoses were supplemented with saline solution to achieve a volume of 50 μL. After theincubation period (21 days), all eggs were opened, LD50 and embryotoxic dose limitswere determined and tissue samples were taken for histopathological examinations.Results and Discussion: In this study, similar mortality rates were found amongstthe control and experimental groups. Although homeopathic agents have beensuggested for the therapy of some problems during pregnancy, the safety of theseproducts in pregnancy is subjective and not scientifically tested. However, eachproduct must be tested. No histologic and macroscopic pathology was determinedin any of the groups in this research. However, homeopathic products can be toxicor safe depending on the dose, source and route of administration. It can be statedthat TCAE (50 μL, 25 μL, 12.5 μL, 6.25 μL, 3.125 μL) does not have an adverse effect onthe viability and organ development of chick embryos at any dose. Hence, it may beaccepted as safe when used in pregnancy.
(2) (PDF) A Tarantula cubensis alkoholos kivonat embriotoxikus hatásának vizsgálata in ovo modellen (Determination of embryotoxic effects of Tarantula cubensis alcoholic extract with in ovo model). Available from: [accessed Jan 10 2023].