The evaluation of the architectural acoustics of the mosques, as one of the religious venues, has been carried out in terms of analyzing the comprehension of the sermon and the preaching, of mawlid and hymn, which have musical characteristics and of the imam's recitation during the performance of the salaat with the religious community. After the settlement of the Turks in Anatolia, especially in the early 13th century, new monuments have been built. In this paper, The Ahi Serafettin Hermitage and Mosque, that embodies the authentic design and the details of the era with it's wooden posts and flat slab ceiling on the interior, has been discussed. Primarily, the reverberation time (T-30) has been analyzed according to the standard of TS EN ISO 3382-1 and the background noise level (LeciA) have been measured. Following that, the parameters of the reverberation time (T-30), the early decay time (EDT), the clarity (C80), the speech transmission index (STI), the definition (D50) and the speech pressure level (SPLA) are defined by using the ODEON (v 10.02) simulation program. As a result of these, an assessment has been made in comparison to the acquired measurements and the simulation data.