Manisa Fault is a geomorphologically distinct normal dip-slip fault, which oversees the southern edge of the Manisa Graben that is a continuum of the Gediz Graben towards the west. This study aims to determine the neotectonic activity of the Manisa Fault and the most recent time of the change in its stress condition through age-dating data obtained by using Th-230/U-234 dating methodology applied on the calcite coating that develops over hanging-wall of the Manisa Fault and the calcite veins that occur as fracture fillings. The age of the calcite precipitations associated with the Manisa Fault was determined to be between 307 +/- 203 and 444 +/- 101ka by using the Th-230/U-234 dating method. Evaluation of the carbonate precipitations on the Manisa Fault along with the age data and the kinematic indicators ascertained that the Manisa Fault switched to a dip-slip normal faulting character from Middle Pleistocene onwards and that the region was under the effect of a NE-SW directional extensional regime. In addition, the opening rate was attempted to be determined using the roll-over anticline structure that advanced depending upon the movement of the fault on the upper horizontal strata of colluviums, which developed in association with the Manisa Fault. Along with the evaluation of the rise in the horizontal stratification in colluvium and the obtained age data, opening rate of the Manisa Fault was determined as 0.01mmy(-1).