Identification of Pectobacterium Isolates Causing Bacterial Soft Rot Disease in White Head Cabbage Production Areas of Yozgat Province


ÖZTÜRK M. , SOYLU S.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, vol.25, no.3, pp.495-503, 2022 (Journal Indexed in ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.18016/ksutarimdoga.vi.943765
  • Title of Journal : KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE
  • Page Numbers: pp.495-503
  • Keywords: Bacterial disease, Soft rot, Pectobacterium, White head cabbage, Yozgat province, CAROTOVORUM SUBSP CAROTOVORUM, STRAINS CAUSING BLACKLEG, VAR CAPITATA L, ERWINIA-CAROTOVORA, 1ST REPORT, STEM ROT, CAPSICUM-ANNUUM, POTATO, PCR, ATROSEPTICUM

Abstract

During the 2018-2019 vegetation period, survey was conducted to ident ify soft rot disease agent in the white head cabbage production areas of Yozgat. Crystal Violet Pectate (CVP) medium was used for the isolation of bacterial agent from tissues showing watery-wet lesions and rotting signs on leaves. stems and roots. A total of 16 pectolytic isolates that formed cavity in the CAT medium were obtained from 24 suspicious plant samples collected from different fields. The colonies of bacterial isolates detected as transparent. convex and crater with edges were gram negative. facultative anaerobe, oxidise negative. catalase positive. and did not show fluorescence on the King B-medium under UV-light. It has been determined that the isolat es caused soft rot symptoms in potato slices and cabbage plants could grow at 37 degrees C, had turbidity in liquid medium containing 5% NaCl and cause hypersensitivity reaction in tobacco leaves. In PCR analyses with obtained 16 isolates revealed that all of the isolates general NI 434 bp and 550 bp PCR product using Y1/Y2 and EXPCCF/EXCPCCR primers which were recommended for the identification of Pectobacterium spp, and P carotovorum subsp. carotovorum. respectively. Based on biochemical, physiological, pathogenicity and molecular analysis, 16 isolates were identified as P carotovorum subsp. carotovorum. It has been also observed that all pathogenic isolates caused signs of soft rot in the form of watery wet lesions in artificially inoculated cabbage plants. It was determined for the first time with this study that. P. carotovorum subsp. carotovorum is the source of soft rot disease infection in the white head cabbage production areas of Yozgat province.