The ameliorative effects of vinpocetine on apoptosis and HSP-70 expression in testicular torsion in rats


Sonmez M. F. , ÖZDEMİR S., Guzel M., Kaymak E.

BIOTECHNIC & HISTOCHEMISTRY, vol.92, no.2, pp.92-99, 2017 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 92 Issue: 2
  • Publication Date: 2017
  • Doi Number: 10.1080/10520295.2016.1259499
  • Title of Journal : BIOTECHNIC & HISTOCHEMISTRY
  • Page Numbers: pp.92-99
  • Keywords: antioxidant, apoptosis, HSP-70, rat, testicular torsion, vinpocetine, ISCHEMIA-REPERFUSION INJURY, HEAT-SHOCK-PROTEIN, OXIDATIVE STRESS, ISCHEMIA/REPERFUSION INJURY, CARBON-TETRACHLORIDE, TESTIS, TORSION/DETORSION, SPERMATOGENESIS, LIVER, HSP70-2

Abstract

Vinpocetine is a potent antioxidant and free radical scavenger. We investigated the effects of vinpocetine on torsion/detorsion (T/D) induced testicular damage, HSP-70 expression and germ cell apoptosis in rats. Sixty Wistar albino adult male rats were divided into five groups of 12. The groups comprised a control group, a sham treated group, a T/D group, a vinpocetine treated group, and a T/D plus vinpocetine treated group. The left testis of each rat was subjected to unilateral torsion followed by detorsion after 2 h. Vinpocetine was administered intraperitoneally immediately and for 10 days following detorsion. At the end of the study, the rats were sacrificed and their testes removed and processed. HSP-70 expression, apoptosis and histopathological damage scores were determined for each group. Testicular T/D caused significant increases in apoptosis and HSP-70 expression, and a significant decrease in Johnsen's testicular biopsy scores and mean seminiferous tubule diameter. Vinpocetine ameliorated testicular histopathology and HSP-70 expression in the T/D + vinpocetine group. Consequently, vinpocetine may prevent testicular injury following testicular torsion owing to its antioxidant effects.