Abiotic factors affecting in vitro pollen grain germination in sainfoin (Onobrychis viciifolia scop.)

SANCAK C., UZUN S., KHAVAR K. M., ÖZCAN S.

Pakistan Journal of Botany, vol.35, no.4, pp.463-471, 2003 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 4
  • Publication Date: 2003
  • Journal Name: Pakistan Journal of Botany
  • Journal Indexes: Scopus
  • Page Numbers: pp.463-471
  • Yozgat Bozok University Affiliated: Yes

Abstract

A reliable in vitro method for germination of sainfoin pollen grains has been developed. Highest pollen germination rate of 96% with mean pollen tube length of 223.5 μm was obtained on medium containing of 100 g/1 sucrose, 200 mg/1 KNO3, 150 mg/1 H3BO3, 150 mg/1 MgSO4, 150 mg/1 Ca(NO3)2, 175 g/1 PEG 4000 and stigma extract at pH 6.5.