Aim: To evaluate the relationship between pre-biopsy PSA levels, International Prostate Symptom Score (IPSS), maximum urinary flow rate (Qmax), prostate volume and positive core numbers of histologically proved chronic prostatitis in the patients whom underwent transrectal ultrasound guided biopsy for elevated prostate specific antigen (PSA) levels. Material and Method: Between January 2012 and 2014, 152 patients underwent TRUS biopsy. Their medical records are examined retrospectively. The mean age of the patients were 62 (45-75). The pathologic specimens were evaluated for the number of cores with chronic prostatitis and percentage of prostatitis. Pre-biopsy levels of PSA, IPSS, maximum urinary flow rate, prostate volume are compared with the number positive cores for chronic prostatitis and their percentages. These variables also compared in the non-chronic prostatitis patients. Results: There was no statistically significant correlation between the positive number of cores for chronic prostatitis and age (P=0.5, r=0.055), a positive correlation was observed between IPSS (P<0.001, r=0.646), prostate volume (P=0.04, r=0.185), and PSA (P=0.001, r=0,309). There was a statistically negative correlation observed with maximum urinary flow rate (P<0.001, r=-0,628). Discussion: The higher values of IPSS values in chronic prostatitis patients, and lower values of maximum urinary flow rates demonstrated that chronic prostatitis is correlated with lower urinary tract symptoms. Similarly, statistically significant higher PSA levels observed in the chronic prostatitis patients demonstrated the relation of inflammation and PSA like the previous studies in the literature.