A Comparison of Genomic DNA Extraction Protocols in Artemisia annua L. for Large Scale Genetic Analyses Studies

Valizadeh N., Holasou H. A., Mohammadi S. A., KHAVAR K. M.

Iranian Journal of Science and Technology, Transaction A: Science, cilt.45, sa.5, ss.1587-1595, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s40995-021-01170-9
  • Dergi Adı: Iranian Journal of Science and Technology, Transaction A: Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, ABI/INFORM, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Communication Abstracts, zbMATH
  • Sayfa Sayıları: ss.1587-1595
  • Anahtar Kelimeler: Artemisia, DNA extraction, Genetic diversity, Molecular markers
  • Yozgat Bozok Üniversitesi Adresli: Evet

Özet

Artemisia anuua L. is an important economic medicinal plant well known for its bioactive compound artemisinin that is used as an antimalarial drug. Extraction of high-quality genomic DNA suitable for restriction enzyme reactions and PCR amplifications is very difficult due to the presence of high amounts of secondary metabolites in A. anuua. This study compared efficiency of Fermentas genomic DNA purification kit (FGDK), phenol–Chloroform, cetyltrimethyl ammonium bromide (CTAB), sodium dodecyl sulfate and Sarkosyl nitrogen methods for extraction of DNA. The quality and quantity DNA samples were determined by physical appearance, agarose gel electrophoresis, spectrophotometer and PCR amplifications. The study used Inter retrotransposon amplified polymorphism (IRAP), retrotransposon-microsatellite amplified polymorphism (REMAP) and start codon targeted-based primers. The results revealed that genomic DNA isolated by CTAB method was comparatively pure and produced clear polymorphic bands compared to the polymorphic bands noted on DNA extractions using other protocols.