Survey of Protein, Amino Acids, and Lipid Profiles in Hempseed Using FTIR Spectroscopy


Alsaleh A., Yilmaz G., Yazici L., Yildirim C., Akdaş O., Khavar K. M.

VIII International Seed Congress 2024, Antalya, Türkiye, 9 - 12 Aralık 2024, ss.1-21

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1-21
  • Yozgat Bozok Üniversitesi Adresli: Evet

Özet

Studying the structures, amino acids, and lipid profiles of hempseed protein is crucial for understanding the nutritional value, functionality, and genetic diversity of Cannabis sativa L. They also inform targeted breeding programs aimed at enhancing desirable traits. Additionally, lipid profiles are essential for industrial uses such as biofuels and cosmetics. In contrast, certain amino acids and lipids offer significant health benefits, providing reassurance about the potential impact on human health. This study examines the biochemical and structural characteristics of 24 hempseed genotypes collected in the 2023 season. Using Fourier Transform Infrared spectroscopy (FTIR), we analyzed protein structures (Amide I, II, III), amino acids, and lipid profiles, classifying the genotypes into dioecious and monoecious groups. The results revealed significant structural variations: monoecious seeds showed higher average absorbance for most studied traits, particularly α-helix-rich proteins (0.486 AU) compared to dioecious seeds (0.476 AU), while β-sheet structures were similar (dioecious: 0.428 AU; monoecious: 0.432 AU). Amino acid profiles were notable, with monoecious genotypes having higher tryptophan levels (0.490 AU) than dioecious (0.478 AU). Ester linkages related to triglycerides averaged 0.735 AU for dioecious and 0.719 AU for monoecious groups, highlighting their nutritional significance. Lipid analyses showed intense compositions, averaging 0.642 AU for dioecious and 0.638 AU for monoecious genotypes. Maltepe-4 genotype showed the highest values, indicating strong protein stability. This analysis underscores the genetic diversity among these genotypes and its significant implications for targeted breeding programs that optimize hemp for various applications. Ultimately, these insights will contribute to the sustainable cultivation of hemp and its diverse uses in the food industry. This research emphasizes the versatility and importance of hempseeds in nutrition and industry.