Akademik Veri Yönetim Sistemi
Revista Cientifica-Facultad de Ciencias Veterinarias, cilt.34, sa.1, ss.1-7, 2024 (SCI-Expanded)
This study was aimed to determine by immunohistochemical methods the expressions of acute phase protein
such as Haptoglobin, C-reactive protein and Serum Amyloid in the lungs of cattle suffering from pneumonic
pasteurellosis (PP). The material of the study was the lungs of 8-18 month old cattle Holstein with Fibrinous
Bronchopneumonia brought for necropsy to the Pathology Department of the Selcuk University Veterinary
Faculty between 2019-2020. As a result of microbiological culture from the lungs of these animals, 6 lungs
where Pasteurella spp. was isolated were used in the study. Additionally, 6 culture-negative healthy animals
obtained from the nearby slaughterhouse were used for lung control. A total of 12 bovine lung samples were
subjected to histopathological and immunohistochemical staining. In the histopathological examination, while
no microscopic lesions were observed in the lungs of the control group, inflammatory hyperemia and red and
grey consolidation stages of fibrinous pneumonia were detected in animals with pneumonic pasteurellosis.
Immunohistochemically, immunoreactivity was very mild or without staining for primers in healthy lungs.
Animals with pneumonic pasteurellosis showed immunopositivity in haptoglobin (Hp), oedema fluid and
inflammatory cell infiltrations (P>0.05). C-reactive protein (CRP) staining showed expression in all criteria
(epithelial, capillary, oedema and inflammatory cell infiltration) at all phases, and the highest mean score was
found during the red consolidation phase (P<0.01). Serum Amyloid A (SAA) staining showed immunopositive
staining in oedema fluid and inflammatory cell infiltrations, and the highest mean scores were during the red
and grey consolidation phases (P<0.01). According to the results of the study, it can be considered that acute
phase proteins may be important in the development process and/or pathophysiology of the disease, and SAA
and CRP may provide more sensitive results for the diagnosis and prognosis of the disease.