Akademik Veri Yönetim Sistemi
INTERNATIONAL INTERDISCIPLINARY CONGRESS OF WOMEN IN SCIENCE, Sivas, Türkiye, 13 - 14 Ocak 2023, ss.12-16
Medicinal plants contain secondary metabolites with important biological activities and have
been used in the treatment of various diseases for years. Origanum vulgare is one of the
traditional spice plants used as a flavouring in foods. Moreover, O. vulgare has been used in
cosmetic and medicinal industries. The flavouring effect of O. vulgare is due to the bioactive
compound contents and the high percentage of essential oil content. Its essential Carvacrol
and thymol are the major essential oil compounds of O. vulgare. Rosmarinic acid is the main
product of O. vulgare. In this study, cytotoxic effect of ethyl acetate extract of O. vulgare
collected from Gaziosmanpaşa University Medicinal and Aromatic Plants Garden has been
researched for cytotoxicity by MTT [3- (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium
bromide] assay using mouse fibroblast cell lines (L929), Human breast cancer cell lines
(MDA-MB-231) and Colorectal carcinoma cell lines (DiFi). To calculate the percentage of
viable cells, absorbance was measured at 570 nm with the Elisa plate reader. Treatment of O.
vulgare ethyl acetate extract with MDA-MB-231 cell lines resulted in 69% viability at a
concentration of 10 μg/mL. In concerning the DIFI cells, the viability was observed as 26%
after the treatment of O. vulgare ethyl acetate extract with the DIFI cell lines at 10 μg/mL. In
L929 cell lines which is normal cells, the viability was determined as 75% at 10 μg/mL. As a
result, O.vulgare could be a promising drug candidate for colorectal carcinoma.