Environmentally friendly synthesis and biological activity assessment of titanium dioxide nanoparticles with hemp (Cannabis sativa L.) leaf extract

KORKMAZ N., Ceylan Y., Kısa D., GÜÇLÜ E., Şen F., KARADAĞ A.

Next Materials, vol.9, 2025 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 9
  • Publication Date: 2025
  • Doi Number: 10.1016/j.nxmate.2025.101323
  • Journal Name: Next Materials
  • Journal Indexes: Scopus
  • Keywords: Biological activity, Green TiO2-Nanoparticle, Hemp (Cannabis sativa L.), TiO2, Toxic
  • Yozgat Bozok University Affiliated: Yes

Abstract

This study investigates the synthesis of titanium dioxide nanoparticles (TiO₂-NPs) using extracts from Cannabis sativa L. leaves. FT-IR analysis identified phytochemicals involved in the synthesis and stabilization of TiO₂-NPs. XRD revealed the crystalline structure, while SEM showed spherical NPs with diameters of 21–29 nm. EDX provided compositional details. The synthesized TiO₂-NPs exhibited antimicrobial activity, inhibited breast cancer cell proliferation, and had an AChE IC50 of 2.472 mg/mL. Hemp waste leaves, used for the first time as a reducing agent, proved effective in NP synthesis, highlighting the potential of renewable plant resources in nanomaterial production. This study is a good example of the use of edible and sustainable waste plant resources in NP synthesis studies.