Plant regeneration by somatic embryogenesis from immature seeds of Sternbergia candida Mathew et T. Baytop, an endangered endemic plant of Turkey


Parmaksiz I., KHAVAR K. M.

Propagation of Ornamental Plants, vol.6, no.3, pp.128-133, 2006 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 2006
  • Journal Name: Propagation of Ornamental Plants
  • Journal Indexes: Scopus
  • Page Numbers: pp.128-133
  • Keywords: Endemic, Genetic diversity, Immature seeds, In vitro, Multiplication, Sternbergia candida
  • Yozgat Bozok University Affiliated: Yes

Abstract

Tissue culture techniques have been established as a useful approach for in vitro conservation of endemic plant species. This report describes in vitro bulblet production of Sternbergia Candida Mathew et T. Baytop, an endangered plant species endemic to the Mugla and Antalya provinces of Turkey, through somatic embryogenesis from immature seeds. The best embryogenesis was observed on MS medium containing 2 mg l-1 6-benzylaminopurine (BAP) and 0.50 mg l-1 Potassium salt of α-naphthalene acetic acid (NAA). The bulblets were converted to plantlets on MS medium with or without 5 g l -1 active charcoal. The addition of active charcoal to the plant conversion media had a complimentary effect and resulted in faster growth and development of roots in the converted plantlets. These were acclimatized in the green house for transfer to the fields to evaluate flowering behavior and the extent of genetic diversity in the morphology of the plants.