The purpose of this research is to investigate the changes in heart rate, blood lactate and oxygen saturation values of female futsal players during the game. Eleven athletes (Xage: 15,50 +/- 0,53 years, Xheight: 162 +/- 0,17 cm, Xweight: 57,67 +/- 4,11 kg) who are players in a women's futsal team in Denizli participated in the study voluntarily. The players who attended the study performed a match with official rules. Before the game, heart rate and oxygen saturation values of the subjects; heart rate, oxygen saturation and blood lactate values at half time and end of the game were obtained. Heart rate and oxygen saturation values of the subjects were taken from the left hand index finger with the pulse oximeter. Blood lactate values of the subjects were taken from the earlobe with a lactate analyzer at half time and 3 minutes after the end of the game while the subject was sitting. Collection of the blood samples from the ear lobe were made at half-time and 3 minutes after the end of the game. Descriptive analyzes of the test parameters were calculated as mean and standard deviation. Whether the data differed statistically, the normal distribution and homogeneity of the variances were analyzed and analyzed by t-test in dependent groups. There was a significant difference in the heart rate values of the athletes before the game, at half time and after the game (p < 0.05). But, there was no statistically significant difference in the oxygen saturation values of the athletes before the game, at half time and after the game (p > 0.05).