Effects of Cisplatin Electrochemotherapy on Human Neuroblastoma Cells


EŞMEKAYA M. A. , KAYHAN H., COŞKUN A. , Canseven A. G.

JOURNAL OF MEMBRANE BIOLOGY, vol.249, no.5, pp.601-610, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 249 Issue: 5
  • Publication Date: 2016
  • Doi Number: 10.1007/s00232-016-9891-4
  • Title of Journal : JOURNAL OF MEMBRANE BIOLOGY
  • Page Numbers: pp.601-610
  • Keywords: Electroporation, Cisplatin, Neuroblastoma, Cell viability, Apoptosis, TUMOR-CELLS, ELECTROPORATION, ELECTROPERMEABILIZATION, PULSES

Abstract

Electrochemotherapy is the usage of electroporation to introduce chemotherapeutic drugs through membrane pores into target cells for cancer treatment. The effectiveness of chemotherapeutic drugs would be increased dramatically when they are used in electrochemotherapy than standard chemotherapy. In the present study, we investigated the effects of cisplatin treatment with electroporation on human SH-SY5Y neuroblastoma cells. SH-SY5Y cells were treated with different concentrations (0.15-24 A mu g/mL) of cisplatin and then exposed to 1500 volts per centimeter (V/cm), 100 microseconds (A mu s) pulse duration, and 1 Hertz (Hz) electric pulses. Cisplatin alone showed a dose-dependent effect on cell viability. On the other hand, cisplatin + electroporation treatment was more effective than cisplatin treatment alone. Lower doses of cisplatin treatment with electroporation was as effective as higher doses of cisplatin treatment without electroporation. These results indicated that cisplatin cytotoxicity was potentiated after exposure of cells to high intensity electric pulses and low doses of cisplatin can be used with electroporation in the treatment of neuroblastoma.