Adventitious shoot regeneration of the medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell) using different internodes

Karatas M., Aasim M., Dogan M., KHAVAR K. M.

Archives of Biological Sciences, cilt.65, sa.1, ss.297-303, 2013 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.2298/abs1301297k
  • Dergi Adı: Archives of Biological Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.297-303
  • Anahtar Kelimeler: Adventitious shoot regeneration, Aquatic plant, Mass proliferation, Medicinal plant
  • Yozgat Bozok Üniversitesi Adresli: Evet

Özet

Water hyssop (Bacopa monnieri L.) is an important medicinal plant due to its active compounds. The plant is also used in ornamental aquaria mainly due to its appearance and adaptability. This study reports on the adventitious shoot regeneration of water hyssop by culturing different internodes and leaf explants on MS media supplemented with various combinations of BA and NAA. All explants induced calli and shoots on all combinations of BA+NAA. The maximum number of shoots per explant on all explants was observed on MS medium supplemented with 0.25 mg/l BA+0.25 mg/l NAA. A higher concentration of NAA inhibited shoot regeneration with all concentrations of BA. Shoots obtained from leaf explants were longer than those from other explants. Regenerated shoots were successfully rooted on MS medium supplemented with IBA. Rooted plantlets were successfully acclimatized in water of various pH levels between 4.0-10.00. It was found that plants can be established on slightly acidic to slightly alkaline media. However, pH 8.0 was found to be more suitable for plant growth under aquatic conditions.