In this study, wild mustard oil methyl esters were produced from wild mustard oil by transesterification. B20 and B2 type fuels were formed by mixing B100 type biodiesel with diesel fuel in 20% and 2% rates in volume. Fuel properties of fuels (B100, B20 and B2) were determined. The fuels obtained were tested in a four-stroke, 3-cylinder, 60 HP, direct injection diesel engine and were compared with diesel fuel in terms of the torque, power, fuel consumption and fume density. When B100, B20 and B2 fuels were used, the maximum torque was realized at 1200 1/min for all fuel types, B100 showed 2.39% and B20 showed 0.81% increase compared to diesel fuel, while B2 showed the same values. All of the fuels showed maximum power at 2500 1/mm, B100 and B20 showed 5.64% and 2.64% increase, respectively, while B2 didn't show any difference. When B100 used at maximum power, there was 2.86% increase in specific fuel consumption and B20 and B2 fuels showed 1.80% and 2.84% decrease, respectively. In the studies with diesel fuel, B100, B20 and B2 fuels, the highest total efficiency were determined as 34.348% at 1300 1/min, 36.103% at 2000 1/min, 36.911% at 1200 1/min and 34.565% at 1200 1/min, respectively. With increasing mixing rates, exhaust smokiness values at all engine speeds showed higher levels of decreases than diesel fuel.