Aim: Cerebrovascular diseases (CVD) are among the major causes of mortality and morbidity worldwide. Cystatin C is considered as an important risk factor for stroke and its increase is considered to be due to inflammation or alterations in renal functions. The Aim of the present study is to evaluate the role of cystatin C in diagnosis and prognosis of stroke patients. Material and Method: The study group was composed of 52 acute cerebral stroke patients admitted to hospital during first 24 hours of occurrence and 42 age and sex-matched healthy controls. Cystatin C levels were measured in Roche Cobas autoanalyzer by Dako Cytomation LX002 cystatin C reactive with an immunoturbidimetric method. Results: Serum cystatin C, sedimentation (ESR), urea, creatinine, eGFR ve CRP levels were higher in the patients when compared to control group (p<0.001 for all). No significant difference in cystatin C levels among the groups constituted according to NIHSS (North Scandinavian Stroke Scale) score was found. Cystatin C levels were found to be moderately correlated with urea, creatinine, eGFR and ESR levels (p<0.001). Levels of cystatin C were still found to be significant in ischemic stroke patients after the effects of kidney function tests (urea, creatinine, eGFR) and inflammatory marker(CRP, ESR, Fibrinogen) were adjusted (p<0.001). Discussion: Cystatin C levels are elevated in acute ischemic stroke. The analyses made to determine if this increase is caused by inflammation or renal dysfunction showed both processes to be correlated with cystatin C, but cystatin C is also an independent risk factor in acute ischemic stroke.