Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method

Tarhan G., Ceyhan I., ŞİMŞEK H., Tuncer S.

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.39, no.2, pp.253-257, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 2
  • Publication Date: 2009
  • Journal Name: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.253-257
  • Keywords: DNA extraction, Mycobacterium tuberculosis, PCR, POLYMERASE-CHAIN-REACTION, RESPIRATORY SPECIMENS, SAMPLES, AMPLIFICATION, HYBRIDIZATION, INHIBITORS, ASSAY
  • Yozgat Bozok University Affiliated: No

Abstract

Aim: DNA extraction is an important step from clinical samples for molecular diagnosis of tuberculosis by PCR. The aim of this study was to evaluate the efficacy of five DNA extraction methods [boiling, single step proteinase K, GuSCN lysis and izopropanol precipitation (Heliosis, METIS Company, TURKEY). DNA precipitation (Epicentre Technologies), and solid phase absorption (QIAamp DNA mini kit, QIAGEN, Valancia,CA)] in searching Mycobacterium tuberculosis DNA in smear positive sputum samples.