Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method

Tarhan G., Ceyhan I., ŞİMŞEK H., Tuncer S.

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.39, sa.2, ss.253-257, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 2
  • Basım Tarihi: 2009
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.253-257
  • Anahtar Kelimeler: DNA extraction, Mycobacterium tuberculosis, PCR, POLYMERASE-CHAIN-REACTION, RESPIRATORY SPECIMENS, SAMPLES, AMPLIFICATION, HYBRIDIZATION, INHIBITORS, ASSAY
  • Yozgat Bozok Üniversitesi Adresli: Hayır

Özet

Aim: DNA extraction is an important step from clinical samples for molecular diagnosis of tuberculosis by PCR. The aim of this study was to evaluate the efficacy of five DNA extraction methods [boiling, single step proteinase K, GuSCN lysis and izopropanol precipitation (Heliosis, METIS Company, TURKEY). DNA precipitation (Epicentre Technologies), and solid phase absorption (QIAamp DNA mini kit, QIAGEN, Valancia,CA)] in searching Mycobacterium tuberculosis DNA in smear positive sputum samples.