Development of a modified method to extract dsRNA from different plant host species

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Delpasand Khabbazi A., Delpasand Khabbazı S.

International Molecular Plant Protection Congress,, Adana, Turkey, 10 - 13 April 2019, pp.104

• Publication Type: Conference Paper / Summary Text
• City: Adana
• Country: Turkey
• Page Numbers: pp.104
• Yozgat Bozok University Affiliated: Yes

Abstract

Viruses are among the important pathogens causing substantial losses in horticultural crops. The first step in virus studies is to identify the virus type. The stable structure of double stranded RNA (dsRNA) in comparison to RNA, allows the molecular detection studies to use dsRNA in virus detection. It is demonstrated that the plant type and species affect the dsRNA isolation. In this study, we collected different herbaceous and woody plants including Vitis vinifera, Gomphrena globose, Phaseolus vulgaris and Rosa kordessi infected with different viruses (Grapevine fanleaf virus, Cucumber mosaic virus, Peanut stunt virus and Prunus necrotic ring spot virus), to evaluate the versatility of the method. The affinity of nucleic acids for the cellulose CF-11 in 1X STE buffer with 16% ethanol comprises the base of this modified protocol. Being a cost effective as well as simplicity make this method advisable for plant molecular pathology and virus studies.