Investigation of the effect of yarrow (Achillea millefolium) on Ehrlich ascites tumor
Postepy Higieny i Medycyny Doswiadczalnej, vol.76, no.1, pp.431-443, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 76 Issue: 1
- Publication Date: 2022
- Doi Number: 10.2478/ahem-2022-0028
- Journal Name: Postepy Higieny i Medycyny Doswiadczalnej
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Directory of Open Access Journals
- Page Numbers: pp.431-443
- Keywords: animal, apoptosis, cell, EAT, yarrow
- Open Archive Collection: AVESIS Open Access Collection
- Yozgat Bozok University Affiliated: Yes
Abstract
Introduction. One of the most important health problems today is cancer. The aim of this study was to investigate the in vitro effect of yarrow (Y) with known anticarcinogenic effect on Ehrlich ascites tumor (EAT). Materials and Methods. The above-ground part (300 g) of Y was macerated with water and extracted three times for 24 hours at 37ºC in a shaking water bath. In the study, EAT cells were divided into control, DMSO group 5-FU, 50, 100, 200, 400 and 800 µg/ml YP groups. Results. At the end of the hour, it was observed that total apoptosis increased significantly in Y groups (especially 50 µg/ml) compared to the control group (p<0.05). It was observed that Y slowed the division of EAT cells (especially 800 µg/ml) by stopping the cell cycle at the G0/G1 stage. It was concluded that Y (especially at high doses) triggered apoptosis by significantly increasing the percentage of total depolarized cells (p<0.001) in all three time periods. Conclusions. The results obtained showed that Y extract may have an antitumoral effect on EAT cells. It is thought that this study will contribute to studies on cancer treatment.