In silico detection of Cucurbitacin-E on antioxidant enzymes of model organism Galleria mellonella L. (Lepidoptera: Pyralidae) and variation of antioxidant enzyme activities and lipid peroxidation in treated larvae

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ERCAN F., BAŞ H., YALÇIN AZARKAN S.

BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES, cilt.11, sa.1, 2022 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1186/s43088-022-00310-3
  • Dergi Adı: BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Anahtar Kelimeler: Ecballium elaterium, Galleria mellonella, Antioxidant enzyme, Pest control, ECBALLIUM-ELATERIUM L, SQUIRTING CUCUMBER, FUMIGANT TOXICITY, PROTECTIVE ROLE, ESSENTIAL OIL, ACID, GLUTATHIONE, LYCOPENE, EXTRACT, TISSUES
  • Yozgat Bozok Üniversitesi Adresli: Evet

Özet

Background: In silico studies further provided predictive binding properties of selected ligands for inhibition of target protein. In the study, molecular binding poses of Cucurbitacin-E and antioxidant enzymes (glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and acetylcholinesterase (AChE) of Galleria mellonella were determined in silico. Cucurbitacins are the most important components of Ecballium elaterium. The first cucurbitacin isolated from the plant was Cucurbitacin-E. In this study, the toxic effect of E. elaterium (L.) A. Rich. (Cucurbitaceae) fruit juice on G. mellonella (Lepidoptera: Pyralidae) larvae, which is known as a good model insect, was also detected, and its effect on antioxidant enzyme activities and lipid peroxidation was revealed.