Akademik Veri Yönetim Sistemi
JOURNAL OF PEST SCIENCE, cilt.87, sa.1, ss.79-87, 2014 (SCI-Expanded)
Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) is cosmopolitan pest of stored products. The effect of strong magnetic fields (MFs) on DNA damage and oxidative stress on larvae stage of E. kuehniella was assessed. Antioxidant enzyme systems, which include superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and malondialdehyde (MDA), the end product of lipid peroxidation as a result of strong MF intoxication that might occur in the larvae tissue, were evaluated. A simple technique of single-cell gel electrophoresis (DNA comet assay) enabled a quick detection of MF treatment on larvae. The larvae were exposed in a 1.4-Tesla (T) MF from a DC power supply at 50 Hz for different time periods (3, 6, 12, 24, 48, and 72 h). MFs caused increasing DNA damage and demonstrated using the comet assay with its parameters including tail DNA%, tail length and tail moment. DNA damage at increasing exposure times were significantly larger than the control group (p < 0.05). These parameters were detected using BS 200 ProP with image analysis software. SOD, CAT, GPx, and GST activities decreased and MDA level increased in the MF-treated group in larvae tissue compared to control group for increasing exposure times at 1.4 T (p < 0.05). In our investigation, we showed that MFs caused oxidative stress and proved to be DNA damage as revealed by the comet assay. MFs may be used to determine potential toxic effects as a control agent against E. kuehniella larvae.