Biodiesel has been produced from various vegetable oils, waste vegetable oils, animal fats and algae oils. This study evaluated the production of biodiesel using sunflower oil (SO), waste sunflower oil (WSO) and neutralized waste sunflower oil (NWSO) which was produced from WSO by neutralization process as main feedstocks. Biodiesels were produced by transesterification of SO, WSO and NWSO with methanolin the presence of a catalyst sodium hydroxide (NaOH). The fuel properties such as density, specific gravity (t/t), kinematic viscosity, flash point, water content, calorific value and copper strip corrosion were determined and discussed with EN 14214 biodiesel standard. Not only were the properties of pure biodiesels but also oils analyzed. Biodiesel produced from SO, WSO and NWSO is an acceptable alternative for diesel fuel and they are possible to use sunflower oil biodiesel (SOB) (pure), waste sunflower oil biodiesel (WSOB) (blend with diesel fuel) and neutralized waste sunflower oil biodiesel (NWSOB) (blend with diesel fuel) in compression ignition engines without any major modification.