The aim of this study was to determine the chemical characterization and to assess full-thickness-excised wound-healing activity of the oil of Pistacia lentiscus L. fruits grown in Algeria. The fatty acid composition of P. lentiscus L. extracts were analyzed by GC-MS and their total protein amounts were determined by elemental analysis. Total thickness-excised wound-healing activity of hexane extract was carried out on rabbits; comparing oil and wax effects. The results showed that P. lentiscus L. fruits contain considerable levels of oil and protein. Additionally, oleic (46.91 +/- 1.03, 48.34 +/- 0.07) in hexane and cold pressure extracts, respectively, palmitic (25.75 +/- 0.82, 25.44 +/- 0.65) and linoleic (20.59 +/- 0.03, 20.27 +/- 0.81) acids were the major fatty acids. Wound-healing activity was significantly higher in P. lentiscus L. fatty oil compared to the wax, therefore, fats refining is wildly recommended.