The Effect of Sample Amount and Decoction Time on The Phytochemicals and Antioxidant Activities of Decoction Lemon Balm and Sage

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Yaman C.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, vol.24, no.4, pp.725-732, 2021 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.18016/
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.725-732
  • Keywords: Decoction, Melissa officinalis, Mineral, Salvia officinalis, Phenolics, MELISSA-OFFICINALIS L., IN-VITRO ANTIOXIDANT, PHENOLIC-COMPOUNDS, INFUSIONS, TEAS, PRODUCTS, CAPACITY, PLANTS, HERBS
  • Yozgat Bozok University Affiliated: Yes


In this study, decoctions (herbal tea) of sage (Salvia officinalis L.) and lemon balm ( Melissa officinalis L), two of the important medicinal plants, were analysed for antioxidant activity, antioxidants, and minerals (K, Na, Mg, Ca, Fe, Ba, Ag and Ga) were determined in each sage and lemon balm dry leaves as well as decoctions by inductively coupled plasma-mass spectrometry (ICP-MS). The antioxidant activities of decoctions were evaluated by scavenging activities against 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and hydrogen peroxide (H2O2) radicals, and varied from 19.4 to 109.1 mg trolox equivalent (TE) cup(-1) for DPPH and from 35.0 to 168.0 mg ascorbic acid equivalent (AAE) cup(-1) for H2O2. The highest antioxidant activity and antioxidants such as total phenolic, flavonoid and flavanol contents were found in the decoction of lemon balm. The analysed minerals were most efficiently observed in sage decoctions. The study also showed that the best sample amount in terms of the minerals was 3 g for both species, but decoction time was statistically insignificant for sage and 10 min more effective for lemon balm. As a result, it was tried to determine the most appropriate sample amount and decoction time for the decoctions of both species according to the analysed parameters.