The aim of this study is to compare some blood biochemical parameters in hemodialysis (HD) patients treated with different dialyser filters. In 70 hemodialysis patients, in six-month period, the monthly retrospective analysis of blood biochemical values has been studied. The differences in urea input/output, creatine input/output and phosphorus (P) levels were evaluated. The dialyser blood flow rate was found to be between 280 to 300 ml/min and dialysate flow rate was 500 ml/min. We found a decrease in urea and creatine levels. At the end of six-month, the most widely used dialyser was 1.5 L (50.8% of total used dialyser), followed by 1.7 L (19.4% of total use) and 1.4 L (17.4% of total use). The least used dialysers were 1.3 L (0.5% of total use used dialyser) and 1.2 L (1.3% of total use used dialyser). The average phosphorus (P) levels were different among the filters 1.4, 1.7 and 2.1 L (P < 0.05) and between 1.5 and 2.1 L (P < 0.05). We found 87.6% decrease in urea and creatine, which was associated with only 3 filters (1.4, 1.5 and 1.7 L). In conclusion, the frequently used dialyser filter was 1.5 L in our center, but we observed that 1.3 L was more effective in decreasing creatine and urea levels. On the other, even though we observed difference in phosphorus levels, this difference might be multifactorial and unrelated to filters used.