Phytochemical profile, antioxidant, antiproliferative, and enzyme inhibition-docking analyses of Salvia ekimiana Celep & Dogan


ŞEKER KARATOPRAK G., GÖGER F., ÇELİK İ., BUDAK Ü. , AKKOL E., Aschner M.

SOUTH AFRICAN JOURNAL OF BOTANY, vol.146, pp.36-47, 2022 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 146
  • Publication Date: 2022
  • Doi Number: 10.1016/j.sajb.2021.09.033
  • Title of Journal : SOUTH AFRICAN JOURNAL OF BOTANY
  • Page Numbers: pp.36-47
  • Keywords: Salvia ekimiana, Antioxidant, Enzyme inhibition, Molecular docking, Cytotoxicity, LC-MS/MS, IN-VITRO ANTIOXIDANT, PHENOLIC COMPOSITION, CHEMICAL-COMPOSITION, ELASTASE ACTIVITY, ESSENTIAL OIL, HPLC-DAD, COLLAGENASE, EXTRACTS, L., IDENTIFICATION

Abstract

Salvia ekimiana Celep & Dogan is an endemic species of Turkey. To our knowledge, the number of studies on biological activities and phytochemical profiling of this plant is quite limited. Therefore, this study aimed to analyze its activities and phytochemical content in detail. The qualitative-quantitative compositions were determined via spectrophotometric and chromatographic (LC-MS/MS and HPLC) techniques. 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH center dot) and 2,2'-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS(center dot+)) radical scavenging and ascorbate-iron (III)-catalyzed phospholipid peroxidation experiments were performed to measure antioxidant capacity. Hyaluronidase, collagenase, and elastase enzyme inhibition tests were determined in vitro using a spectrophotometer. Antiproliferative activity was evaluated in human lung cancer (A549) and human breast cancer (MCF7) cells. The murine fibroblast (L929) cell line was used as a normal control cell. While the subextract rich in phenolic compounds was n-butanol extract, rosmarinic acid was defined as the main secondary metabolite. The highest antioxidant activity observed for the n-butanol subextract included the following: DPPH center dot EC50: 0.08 +/- 0.00 mg/mL, TEAC/ABTS: 2.19 +/- 0.09 mmol/L Trolox, MDA EC50: 0.42 +/- 0.03 mg/mL. The methanolic extract, the ethyl acetate, and n-butanol subextracts displayed significant inhibitory activity on collagenase, while the other subextracts did not show any inhibitory activity on hyaluronidase and elastase. Due to strong interactions with their active sites, molecular docking showed luteolin 7-glucuronide, apigenin 7-glucuronide, and luteolin 5-glucoside had the highest binding affinity with target enzymes. The chloroform subextract showed significant cytotoxicity in all cell lines. These novel results revealed that S. ekimiana has strong antioxidant, collagenase enzyme inhibitory, and cytotoxic potential. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.