© 2019 American Institute of Physics Inc. All Rights Reserved.Novel chemical modification of a natural polymer (soluble eggshell membrane protein (SEP)) with one of carbon nanostructures (graphene oxide (GO)) was used to prepare nano adsorption material. The graphene oxide-soluble eggshell membrane protein (GO-SEP) was characterized using FTIR, XRD, TG, DTG, DSC, SEM, STEM and particle size distribution thereafter was used for packing the solid phase extraction column. The SPE using GO-SEP as packing material was developed and enhanced by unique method depends on using dithiocarbamate as a chelating agent for the lead to determine its concentration in four different water samples by inductively coupled plasma - optical emission spectrometry (ICP-OES). The prepared chelating agent bis sodium barbituric acid dithiocarbamate (BADTC) was produced by treating barbituric acid with CS2 in the presence NaOH. The factors that influence the preconcentration and determination of lead (pH, sample and eluent flow rates, concentration and type of eluents, a volume of sample, chelating agent concentration and interfering ions) have been examined in detail. Detection limits (LOD) (0.1μg.L-1) and the relative standard deviation (RSD%) were found lower than <0.12% (n=10, c=0.5μg.L-1). This new method has been successfully applied to determine lead in four water samples.