Comparative effects of autologous cytokine-rich serum and platelet-rich fibrin on bone regeneration in a rabbit calvarial defect model
Tissue and Cell, cilt.104, 2027 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 104
- Basım Tarihi: 2027
- Doi Numarası: 10.1016/j.tice.2026.104053
- Dergi Adı: Tissue and Cell
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE, Zoological Record
- Anahtar Kelimeler: Autologous cytokine-rich serum (ACRS), Bone defect, Bone regeneration, Platelet-rich fibrin (PRF)
- Yozgat Bozok Üniversitesi Adresli: Evet
Özet
Bone defects from trauma, infection, or tumor resection cause functional and aesthetic complications when natural healing is insufficient. This study combined autologous cytokine-rich serum (ACRS) and platelet-rich fibrin (PRF) with a xenograft to evaluate the early phase of bone regeneration in a rabbit calvarial defect model. Four standardized calvarial defects (9 mm in diameter, 3 mm in depth) were created in each of twelve adult male New Zealand White rabbits and allocated to ACRS with xenograft, PRF with xenograft, xenograft alone, or untreated control. Rabbits were euthanized at weeks 1, 2, and 4 for histopathological and immunohistochemical analyses of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL), runt-related transcription factor 2 (RUNX2), and Ki-67; intergroup comparisons were conducted using within-animal Friedman tests. OPG differed significantly at week 2 (p = 0.027), was lower in ACRS and lowest in PRF, and increased over time in ACRS (p = 0.042) and PRF (p = 0.049), peaking at week 4. RANKL showed no significant intergroup differences; only the control showed a temporal change (p = 0.046). RUNX2 increased significantly in ACRS (p = 0.029) and showed a non-significant trend in PRF (p = 0.060), peaking at week 4. Ki-67 differed significantly at all time points (p = 0.029, 0.007, and 0.007) and was highest in ACRS and lowest in control. ACRS may promote early proliferative activity while PRF may support a more gradual osteogenic response; these findings, limited to the early (4-week) healing period, are exploratory and hypothesis-generating.