In this study, the chemical composition, total phenolic content, and antioxidant activity of essential oils (EOs) obtained from three ecotypes of Ziziphora persica from different locations (Artvin, Gumushane, and Igdir provinces with an altitude of 740 m, 1005 m and 1260 m, respectively) in Turkey were determined. Results showed that Z. persica samples had twenty-two compounds, representing 91.01-92.92% of the essential oils. The pulegone (47.80-61.33%) was identified as the major component in Z. persica, followed by limonene (3.05-5.38%), 1,8-cineole (2.20-4.25%), germacrene D (3.21-3.62%), and beta-caryophyllene (2.61-3.03%). The changes in pulegone, limonene, 1,8-cineole, and ,3-caryophyllene content was due to the differences in the altitude. The radical scavenging activity against DPPH radical expressed as IC50 was found between 0.35-0.44 mg/mL and antioxidant activity expressed as EC50 in FRAP (Ferric reducing power) assay was in the range of 0.59 and 0.75 mg/mL, respectively. Due to the chemical composition and the biological activities of plant essential oils (phenolic content and antioxidant activity), they could be used in food and cosmetic industries, as well as in the human nutrition and health field.