Integrated Perspectives in Agriculture and Biology: Theory and Practice, Hülya Doğan,Hatice Baş, Editör, Özgür Yayınları, Gaziantep, ss.1-18, 2025
Plant tissue culture is a method that enables the regeneration or propagation
of plant parts or whole plants by culturing plant protoplasts, cells, tissues,
or organs under controlled conditions in vitro. Among these techniques,
anther culture, meristem culture, shoot tip culture, ovule culture, and embryo
culture are widely used in vitro techniques.
Embryo culture, defined as the cultivation of embryos isolated from seeds
or seed primordia of higher plants on sterile nutrient media, is utilized for
purposes such as the rapid development of new varieties, the creation of
varieties more resistant to diseases, pests, and abiotic stresses, and enabling
hybridization efforts hindered by incompatibility between plant genera and
species.
In grapevine, embryo culture is employed for objectives such as developing
new grape varieties, propagating seedless grape cultivars, and creating
varieties resistant to diseases, pests, and diverse ecological conditions.
However, despite extensive research on embryo rescue in viticulture, the
number of newly developed cultivars remains limited. Breeding new grape
varieties through embryo rescue is still a challenging and long-term technique,
requiring a patient and dedicated approach from grape breeders.
This compilation will provide current and comprehensive insights into the
embryo rescue technique in grape breeding, discussing its applications and
practices.