In the present study the determination of the effects of arbuscular mycorrhizal fungi on antioxidant enzyme activities and some biochemical changes under drought stress on Kober 5 BB and 110 R grapevine rootstocks was aimed. Cuttings were planted into the media containing perlite and turf (1:1) and irrigated regularly. After one-month mycorrhizal fungi as a liquid formulated were applied at 0 (control), 10 and 20 g/L concentrations. The cuttings have been kept for approximately one months and then they were subjected to drought stress. After one-month treatment injury degree, shoot length, shoot weight, average leaf number per shoot, leaf area, rooting ratio, root length, number of roots and membrane injury, leaf proportional water content, chlorophyll, proline, lipid peroxidation, hydrogen peroxide, total phenolic compound, soluble protein content and antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX)) were determined. As a conclusion, it was demonstrated that mycorrhizal fungi had positively effect on plant development, biochemical characters and antioxidant enzyme activities of grapevine rootstocks in drought stress conditions.