Improvement of Viral dsRNA Isolation from Infected Plants and Vector Nematodes


Delpasand Khabbazi A., Delpasand Khabbazi S.

LAP Lambert Academic Publishing, Beau Bassin-Rose Hill, 2017

  • Yayın Türü: Kitap / Ders Kitabı
  • Basım Tarihi: 2017
  • Yayınevi: LAP Lambert Academic Publishing
  • Basıldığı Şehir: Beau Bassin-Rose Hill
  • Yozgat Bozok Üniversitesi Adresli: Evet

Özet

To control the plant viral diseases, identification of the virus species is considered as a first step. Considering the stable structure of dsRNA in comparison to RNA molecules, dsRNA extraction is a promising tool to elucidate the viral genome sequence. Majority of the RNA viruses produce dsRNA segments as the copies of virus genome; these high molecular weight molecules can be employed in virus identification. To date, several efforts have been made to extract dsRNA from many different plant species. Developing a method charging less and completing fast has been investigated in this study.