Improvement of Viral dsRNA Isolation from Infected Plants and Vector Nematodes

Delpasand Khabbazi A., Delpasand Khabbazi S.

LAP Lambert Academic Publishing, Beau Bassin-Rose Hill, 2017

  • Publication Type: Book / Textbook
  • Publication Date: 2017
  • Publisher: LAP Lambert Academic Publishing
  • City: Beau Bassin-Rose Hill
  • Yozgat Bozok University Affiliated: Yes


To control the plant viral diseases, identification of the virus species is considered as a first step. Considering the stable structure of dsRNA in comparison to RNA molecules, dsRNA extraction is a promising tool to elucidate the viral genome sequence. Majority of the RNA viruses produce dsRNA segments as the copies of virus genome; these high molecular weight molecules can be employed in virus identification. To date, several efforts have been made to extract dsRNA from many different plant species. Developing a method charging less and completing fast has been investigated in this study.