Physalis peruviana L. is belongs to Solanaceae family and commonly known as Cape gooseberry. More recently it is very popular and widely used as medicinal plant to treat malaria, asthma, hepatitis, dermatitis and rheumatism and has diuretic and antiinflammatory properties. In this study, it was aimed to develop in vitro propagation protocol for P. peruviana L. using apical shoots as an explant sources. Regenerated plants were evaluated based on their multiplication rate and shoot length using various concentration of BAP (1, 2, 3 mg l(-1)) in combination with IBA (0, 0.1, 0.2, 0.4 mg l(-1)) and NAA (0, 0.1, 0.2, 0.4 mg l(-1)). In addition, efficiency of various auxin concentrations of (1 and 2 mg l(-1) IBA and NAA) was also applied on root formation of P. peruviana L. The highest shoot numbers were obtained from 2 mg l(-1) BAP with 0.4 mg l(-1) IBA (6.00) combinations 'and shoot length obtained in 2 mg l(-1) BAP with 0.2 mg 14 IBA combinations (3.30 cm). As for the effects of BAP and NAA combinations; the highest shoot length were obtained from 2 mg l(-1) BAP without NAA combinations (3.33 cm) while the lowest one was in 3 mg l(-1) BAP with 0.4 mg/I NAA combinations. The highest root numbers were obtained from NAA application (2 mg l(-1) and 1 mg l(-1), respectively). In vitro derived plants were acclimatized to the soil smoothly. The present study highlights the importance of plant tissue culture in order to be used for large-scale production of P. peruviana (L.) due to the elimination of sexual propagation.