Purpose: The aim of this study is to investigate the effects of preoperative oral intake of liquid carbohydrate on postoperative stress parameters (blood glucose, insulin resistance, cortisol, noradrenaline, and adrenaline levels) in patients who underwent laparoscopic cholecystectomy. Design: This is an experimental study with intervention and control groups. Methods: The sample consisted of 68 patients who underwent laparoscopic cholecystectomy (control group = 33; intervention group = 35). Twelve-hour preoperative fasting was applied to the patients in the control group in accordance with the clinical routine. Clear oral liquid carbohydrate (400 mL; 12.5 g/ 100 mL maltodextrin, 50 kcal/100 mL, pH 5.0) was administered to the patients in the intervention group at the preoperative second hour. Blood samples were taken from the patients at the preoperative 2nd and postoperative 2nd and 24th hours, and their blood glucose, insulin resistance, cortisol, noradrenaline, and adrenaline levels were measured. Results: Preoperative oral intake of carbohydrate had no effect on blood glucose (P > .05) but decreased insulin resistance at the postoperative 24th hour (P = .044; intervention and control group: 3.62 +/- 3.44 to 8.16 +/- 12.57 respectively) and cortisol level at the postoperative 2nd hour (P =.005; intervention and control group: 15.16 +/- 6.53 mg/dl to 20.14 +/- 7.49 mg/dl, respectively). In all of the three measurements, we found that the noradrenaline level of the patients in the intervention group was higher than the value of those in the control group (319.80 +/- 301.49 pg/mL to 211.65 +/- 141.11 pg/mL [P = .450]; 361.40 +/- 213.50 pg/mL to 216.13 +/- 114.53 [P = .001]; 268.40 +/- 164.04 pg/mL to 196.00 +/- 83.33 pg/ mL [P = .026], respectively). Preoperative oral intake of liquid carbohydrate had no effect on postoperative adrenaline level (P > .05). Conclusions: Oral intake of liquid carbohydrate given at the preoperative 2nd hour decreased postoperative stress response through insulin resistance and cortisol. (c) 2020 American Society of PeriAnesthesia Nurses. Published by Elsevier Inc. All rights reserved.