Asymmetric and symmetric dimethylarginine gingival crevicular fluid levels in periodontitis

Güney Z., KURGAN Ş., ÖNDER C., Mammadov C., SERDAR M. A., GÜNHAN M.

Journal of Periodontal Research, vol.58, no.2, pp.256-261, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 58 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.1111/jre.13087
  • Journal Name: Journal of Periodontal Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.256-261
  • Keywords: gingival crevicular fluid, methylated arginines, periodontitis
  • Yozgat Bozok University Affiliated: Yes


Objective: This study aimed to evaluate the level of ADMA (asymmetric dimethylarginine), SDMA (symmetric dimethylarginine), and IL-1β (Interleukin-1β) in gingival crevicular fluid (GCF) from periodontitis patients and control subjects. Background: ADMA and SDMA are potentially hazardous non-proteinogenic amino acids that limit nitric oxide (NO) synthesis and have many functions in various human disorders. ADMA causes a structural change in nitric oxide synthase, while SDMA blocks arginine cell uptake. Increased plasma ADMA has been widely recognized as a “trigger” initiating impaired NO bioavailability and vascular dysfunction, which ultimately leads to oxidative stress. Methods: Twenty-five patients with periodontitis (P) (Stage III, Grade C, n = 25) and 20 control (C) subjects were included in the study. The IL-1β level of GCF was measured by enzyme immunoassay (ELISA) and ADMA and SDMA by liquid chromatography-mass spectrometry (LC–MS/MS). Results: Periodontitis patients had higher clinical parameters than controls (p <.001). Levels of IL-1β, ADMA and SDMA GCF were statistically significantly higher in group P than in group C (respectively; p =.003, p <.0001, p <.0001). There was no difference in the ADMA/SDMA ratio (p =.312) between the groups. There were significant positive correlations between clinical periodontal parameters and IL-1β, ADMA, and SDMA levels (p <.05). ADMA and SDMA levels were significantly correlated with IL-1β (p <.05). Conclusions: These findings suggest that ADMA and SDMA may be involved in the pathogenesis of the periodontal disease.