Determination of β-lactamase activities and antibiotic susceptibility of some Bacillus strains causing food poisoning


Uraz G., ŞİMŞEK H., Maraş Y.

Drug Metabolism and Drug Interactions, vol.18, no.1, pp.69-77, 2001 (Scopus) identifier identifier

  • Publication Type: Article / Review
  • Volume: 18 Issue: 1
  • Publication Date: 2001
  • Doi Number: 10.1515/dmdi.2001.18.1.69
  • Journal Name: Drug Metabolism and Drug Interactions
  • Journal Indexes: Scopus
  • Page Numbers: pp.69-77
  • Keywords: Antibiotic susceptibility test, Bacillus, Inhibitory effect, β-lactamase
  • Yozgat Bozok University Affiliated: Yes

Abstract

Some Bacillus species are important food pathogens. For example, B. cereus is an opportunistic pathogen found in raw milk that is a common cause of food poisoning. It is of interest to investigate the virulant profiles of Bacillus strains isolated from foods and samples associated with food-poisoning outbreaks. Nineteen Bacillus strains were isolated from various milk samples. β-Lactamase enzyme activities of these Bacillus strains were evaluated with iodometric and chromogenic cephalosporin (nitrocefin) test methods. Five of 19 Bacillus strains isolated were positive for β-lactamase activity. Clavulanate-amoxycillin and cephazolin were chosen to test the antibiotic susceptibilities of the β-lactamase positive and negative Bacillus strains. Of the five β-lactamase positive Bacillus strains, three were susceptible, and two strains intermediate to clavulanate-amoxycillin; one was susceptible, and four strains were intermediate to cephazolin. None of the β-lactamase positive Bacillus strains was resistant to both antibiotics. Of the 14 β-lactamase negative strains, five were susceptible to clavulanate-amoxycillin, four strains were intermediate, and five strains were resistant; three were susceptible, one intermediate, and ten β-lactamase negative strains were resistant to cephazolin.