This methodological study was conducted to determine the Turkish validity-reliability and selectivity-sensitivity of the Premature Infant Pain Profile-Revised Form (PIPP-R). 200 newborn infants with gestational age of 26-42 weeks followed up in the neonatal unit of a university hospital. Necessary permissions were obtained from the responsible author, the institution, and the ethics committee before starting the study. Language validity, content validity, and construct validity studies were conducted for the validity of PIPP-R. For the content validity, the opinions of 10 experts were asked and the content validity index was found as 0.88. The factor analysis method was used to determine the construct validity of the scale and it was determined that the PIPP-R consisted of three factors. The Cronbach's alpha coefficient from the internal consistency analysis was calculated for the reliability of the scale and the scale was determined to be highly reliable with the value of 0.840. The intra-class correlation coefficient was used in determining the observer reliability and the agreement between three observers was found to be very good (0.944-1.000). In the analyses conducted for the sensitivity and selectivity of the scale, it was determined that the sensitivity of the scale was 91% and its selectivity was 88%. In accordance with the obtained results, PIPP-R was determined to be a valid, reliable, selective, and sensitive measurement tool to be used for the pain assessment of the Turkish population. (c) 2019 Elsevier Inc. All rights reserved.