Karyotypic characters, mitotic metaphase chromosomes, monoploid ideogram and karyogram of Zygoribatula cognata (Oudemans) (Acari: Oribatida: Oribatulidae) were investigated for the first time. The chromosome number of Zygoribatula cognata was 2n = 30. The chromosomes were holocentric characterized with lack a localized centromere. No satellite was observed in the chromosomes. The sex chromosomes and sex determination of Zygoribatula cognata could not be determined.