Hairy root transformation in turkish chickpea (cicer arietinum l) cultivars
Biotechnology and Biotechnological Equipment, vol.18, no.3, pp.51-54, 2004 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 18 Issue: 3
- Publication Date: 2004
- Doi Number: 10.1080/13102818.2004.10817120
- Journal Name: Biotechnology and Biotechnological Equipment
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.51-54
- Yozgat Bozok University Affiliated: No
Abstract
Rooting of Cicer arietinum L (Chickpea) cultivars Gökçe, Akçin 91 and Izmir 92 was evaluated using wild type 15834 strain of Agrobacterium rhizogenes by direct inoculation and puncture inoculation with needle. Scoring was done after 6 weeks. Directly inoculated explants showed a callogenic mass instead of hairy roots. There was a considerable difference in the highest frequency of hairy root formation which was achieved on variety Gökçe, in the light (16.67%) and dark (63.33%) after puncture inoculation. Mean transformation frequency ranged from 5 to 80%. Results show a significant difference for transformation from explants cultured in the light or dark. © 2004 Taylor and Francis Group, LLC.