Hairy root transformation in turkish chickpea (cicer arietinum l) cultivars
Biotechnology and Biotechnological Equipment, cilt.18, sa.3, ss.51-54, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 3
- Basım Tarihi: 2004
- Doi Numarası: 10.1080/13102818.2004.10817120
- Dergi Adı: Biotechnology and Biotechnological Equipment
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.51-54
- Yozgat Bozok Üniversitesi Adresli: Hayır
Özet
Rooting of Cicer arietinum L (Chickpea) cultivars Gökçe, Akçin 91 and Izmir 92 was evaluated using wild type 15834 strain of Agrobacterium rhizogenes by direct inoculation and puncture inoculation with needle. Scoring was done after 6 weeks. Directly inoculated explants showed a callogenic mass instead of hairy roots. There was a considerable difference in the highest frequency of hairy root formation which was achieved on variety Gökçe, in the light (16.67%) and dark (63.33%) after puncture inoculation. Mean transformation frequency ranged from 5 to 80%. Results show a significant difference for transformation from explants cultured in the light or dark. © 2004 Taylor and Francis Group, LLC.