Visualization of 45S rDNA and 5S rDNA genes on Onobrychis viciifolia (sainfoin) by Fluorescence in situ Hybridization (FISH)


Aksoy T., Kara Öztürk S. D., Tek A. L.

III. Bitki Islahı ve Genetiği Öğrenci Kongresi, Niğde, Turkey, 27 - 28 May 2021, pp.2

  • Publication Type: Conference Paper / Summary Text
  • City: Niğde
  • Country: Turkey
  • Page Numbers: pp.2
  • Yozgat Bozok University Affiliated: No

Abstract

5S and 18-5.8-26S nuclear ribosomal DNA (rDNA) units are located in most eukaryotes. 5S and 18- 5.8-26S rDNA occur at one or several specific loci and rDNA are highly conserved repetitive sequences. Polymorphism or conservatism of rDNA copy number and chromosomal localization are comparable and viewable. Accordingly ribosomal DNA units are used on interspecific phylogenetic relotionships. Additionaly rDNAs are preferred and useful cytogenetics markers for chromosome analysis. Several agricultarly important cultivated plants which are wheat, barley, rice, cotton etc. are worked for understanding this concept. Sainfoin is a member of fabacaea family which is agriculturally important family. Sainfoin is a fodder crop that is preferred because of not bloating to animals. Also nectar-rich sainfoin has nitrogen fixing capacity like other fabacaea members. Sainfoin is dry and cold resistant and can grow in arid and chalky areas. These characteristics makes it important option for breeding studies. Making sense of genome organization and phylogenetic analysis a cultivar illuminates breeding process. Considering this information, visualization of 45S rDNA and 5S rDNA genes by FISH on sainfoin is studied. In research, Onobrychis viciifolia (sainfoin), Özerbey variety are used for plant material. Research results shows, number of 45S rDNA locus is 6 and the number of 5S rDNA locus is 8 in interphase.