Activation of cannabinoid 2 receptors by JWH-133 protects against ovarian ischemia-reperfusion injury in rats


ONAT T. , DEMİR ÇALTEKİN M. , DOĞANYİĞİT Z. , TÜRKLER C., KAYMAK E. , Kara M., ...More

BIOTECHNIC & HISTOCHEMISTRY, vol.96, no.5, pp.394-400, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 96 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.1080/10520295.2021.1905183
  • Title of Journal : BIOTECHNIC & HISTOCHEMISTRY
  • Page Numbers: pp.394-400
  • Keywords: Cannabinoid 2 receptors, inflammation, ischemia-reperfusion injury, JWH-133, oxidative stress

Abstract

We investigated the efficacy of activated cannabinoid 2 receptors for alleviating ovarian ischemia-reperfusion injury in rats. Female Wistar albino rats were divided randomly into six groups: ischemia-reperfusion (IRG); ischemia-reperfusion + 0.2 mg/kg JWH-133 (JIRG1), ischemia-reperfusion + 1 mg/kg JWH-133 (JIRG2); ischemia-reperfusion + 5 mg/kg JWH-133 (JIRG3); solvent control, and sham control. Ovarian ischemia was established for 3 h followed by reperfusion for 3 h. Ovarian tissue was investigated using histology, immunohistochemistry and biochemistry. Administration of JWH-133 synthetic cannabinoid reduced nuclear factor kappa-B immunoreactivity as well as TUNEL positivity scores and malondialdehyde levels. These reductions were significant in all cases except for the malondialdehyde levels in the 1 mg/kg JWH-133 group. Activation of cannabinoid 2 receptors by JWH-133 reduced ovarian ischemia-reperfusion injury due to its antioxidant and anti-inflammatory effects.