Immobilization of beta-galactosidase on Novel Polymers Having Schiff Bases


Aynaci E., SARI N., TÜMTÜRK H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.39, no.4, pp.259-266, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 4
  • Publication Date: 2011
  • Doi Number: 10.3109/10731199.2011.555837
  • Title of Journal : ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Page Numbers: pp.259-266

Abstract

We have developed a strategy to immobilize beta-galactosidase as a model enzyme by using polymeric supports having Schiff bases, which were prepared from (aminomethyl) polystyrene and 2-phenlyindole-3-carboxaldehyde by condensation. beta-galactosidase was immobilized onto the new polymer supports via covalent bonds. The influence of temperature, pH, reusability, and storage capacity on the free and immobilized beta-galactosidase was investigated. Our results indicate that the (aminomethyl) polystyrene with Schiff bases is most suitable for the immobilization of beta-galactosidase. These kinds of new supports can be used for the immobilization of beta-galactosidase due to their strong storage capacity and reusability.