Plant sterols are a group of steroid alcohols with various bioactive characteristics for human health. Phytosterols inhibit cholesterol absorption in intestines and thus reduce blood cholesterol levels. In present study, grain Campesterol, Stigmasterol and Betasitosterol concentrations of 20 different bread wheat cultivars were determined and the relationships between phytosterols and grain mineral nutrients were identified. Correlations analysis revealed that Campesterol had significant positive correlations with nitrogen (r=0.356**) and manganese (r=0.327*) and significant negative correlations with calcium (r=-0.432*) and zinc (r=-0.424**). Stigmasterol had significant negative correlations with calcium (r=-0.291*) and zinc (r=-0.466**) and significant positive correlations with manganese (r=0.256*). Betasitosterol had significant positive correlations with nitrogen (0.342**) and manganese (0.323*) and significant negative correlations with calcium (-0.387**), zinc (-0.468**) and phosphorus (-0.284*). Principle component analysis (PCA) for grain mineral nutrient elements, Campesterol, Stigmasterol and Betasitosterol concentrations revealed that there were 3 traits (Campesterol, Betasitosterol and nitrogen) under PC1 with a high eigenvalue (>0.70). While phosphorus and zinc had high eigenvalue s under PC2, there were not any traits under PC3 with a high eigenvalue. Only boron had a high eigenvalue under PC4.