Twenty-five wheat genotypes (Triticum aestivum L.) were grown at three locations (Samsun, Amasya and Tokat) in the Middle Black Sea Region of Turkey in order to estimate the broad-sense heritability for grain yield and some agronomic and quality traits. Estimation of the heritability will help to identify selection parameters in our breeding programs for target environments. The heritability for grain yield, test weight, 1000-kernel weight, Zeleny sedimentation, protein content and plant height were 46.05%, 86.88%, 81.82%, 89.13%, 87.45% and 43.69%, respectively. It was found that Zeleny sedimentation was the least affected trait over environments and followed protein content, test weight and 1000-kernel weight. On the other hand, grain yield and plant height were the most affected traits across environmental conditions.